Flow cytometry can be adapted in many ways to measure the fluorescence lifetime.
Below is a list of ways in which our laboratory has (past & present) been developing techniques to easily, sensitively, and with high resolution, measure fluorescence dynamics at the throughput of a flow cytometer.
- Frequency domain (analysis of average lifetime as parameter)
- Frequency domain (sorting based on average lifetime)
- Frequency domain (analysis of multiple lifetimes using multi-frequency)
- Frequency domain (analysis of phase and modulation lifetimes for phasor graphing)
- Frequency domain (sorting cells based on a phase-filtering approach)
- Frequency domain (analysis of very short lifetimes by leveraging aliased frequencies higher than Nyquist)
- Hybrid time domain (analysis with capture of full decay kinetics)
- Non-frequency or time (analysis of average lifetime based on cytometric peak-to-peak analysis)